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Sood, Pankaj
- Effect of Granulosis Virus Infection on Food Consumption and Utilization by Pieris brassicae (Linnaeus)
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Authors
Affiliations
1 Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur 176062, Himachal Pradesh, IN
1 Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur 176062, Himachal Pradesh, IN
Source
Journal of Biological Control, Vol 24, No 1 (2010), Pagination: 65-69Abstract
The early fourth instar larvae of Pieris brassicae were fed on cabbage leaves treated with PbGV at three concentrations (2x107, 1x107 and 2x106 occlusion bodies ml-1) to ascertion the food consumption and its utilization by P. brassicae larvae upon infection under laboratory conditions. It was found that the mean consumption index decreased to the tune of 4.39% at 24 hours to 67.19% at 120 hours over control after PbGV infection. Approximate digestibility also reduced from 4.22 to 80.78% at 24 and 120 hours, respectively over control. Food consumption of virus-treated larvae decreased with passage of time as compared to the healthy larvae. Conversion of ingested and digested food to body substance also reduced in virus infected larvae to different degrees which ranged from 2.77 to 96.04% for ingested food and 4.55 to 92.16% for digested food. The differences among various concentrations with respect to consumption index, approximate digestibility, conversion of ingested and digested food were, however, statistically non-significant. The impact of virus infection on food consumption and utilization by P. brassicae larvae was enhanced with time after infection.Keywords
Nutritional Requirement, Food Consumption, Utilization, PbGV, Pieris brassicae.Full Text
- Molecular Characterization of Pieris brassicae Granulosis Virus (pbGV) from the Himalayan Region of India
Abstract Views :241 |
PDF Views:125
Authors
Affiliations
1 Crop Research Sub Station, CSK Himachal Pradesh Krishi Vishvavidyalaya, Sundernagar, Mandi, Himachal Pradesh - 175 019, IN
2 ICAR Research Complex for Eastern Region Research Centre, Plandu, Ranchi - 834 010, Jharkhand, IN
1 Crop Research Sub Station, CSK Himachal Pradesh Krishi Vishvavidyalaya, Sundernagar, Mandi, Himachal Pradesh - 175 019, IN
2 ICAR Research Complex for Eastern Region Research Centre, Plandu, Ranchi - 834 010, Jharkhand, IN
Source
Journal of Biological Control, Vol 26, No 2 (2012), Pagination: 37-43Abstract
A strain of granulosis virus from Pieris brassicae was isolated from the dry temperate region of Himachal Pradesh, India situated at an altitude of 2580 m above msl. The molecular characterization of this strain of PbGV was carried out with granulin gene nucleotide sequence analysis. The nucleotide sequence of 404 bp of PbGV was submitted to GenBank, NCBI with accession number FJ151541. Nucleotide and phylogenetic analysis confirm this isolate as Pieridae (insect family) infecting granulovirus with lowest genetic distance of 0.012, 0.015, 0.016 with other Pieris rapae and Pieris brassicae granulosis viruses. More number of isolates and other molecular markers, however, would be useful to understand the phylogenetic relationship of this Indian isolate of PbGV.Keywords
Granulosis Virus, Pieris brassicae, Granulin and Phylogeny.Full Text
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- Molecular Diversity and Antibiotic Sensitivity of Gut Bacterial Symbionts of Fruit Fly, Bactrocera tau Walker
Abstract Views :242 |
PDF Views:145
Authors
Affiliations
1 Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur 176062, Himachal Pradesh, IN
1 Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur 176062, Himachal Pradesh, IN
Source
Journal of Biological Control, Vol 23, No 3 (2009), Pagination: 213-220Abstract
Random amplified polymorphic DNA-Repetitive extragenic palindromic (RAPD- REP) PCR-restriction length polymorphism (PCR-RFLP) were used to study the genetic similarity among bacterial symbionts of fruit fly, Bactrocera tau Walker. RAPD-PCR and REP-PCR placed three bacteria into two groups, viz., group A (BC1 and BC3) and group B (BC2). However, PCR- RFLP analysis of recA and rrs genes of bacterial symbiont placed BC1 and BC2 into group A and BC3 into group B. Studies on antibiotic sensitivity pattern also supported the results of PCR-RFLP as the response of BC1 and BC2 was similar to different antibiotics. All the bacterial symboints were found to be sensitive to co-trimoxazole, gentamycene and nofloxacin. The findings suggest that there may be a possibility of utilising these antibiotics for disruption of fruit fly biology as foliar symbioticides for their management.Keywords
Bacterial Symbionts, Bactrocera tau, recA gene, rrs gene, RAPD and REP–PCR Analysis.Full Text
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